Development of a cps-based multiplex PCR for typing of Actinobacillus pleuropneumoniae serotypes 1, 2 and 5.
نویسنده
چکیده
A cps-based multiplex PCR for typing of Actinobacillus pleuropneumoniae serotypes 1, 2 and 5 was developed. This method should be specific and practical in Japan where more than 88% of isolates are serotypes 1, 2 or 5.
منابع مشابه
Evaluation of a multiplex PCR test for simultaneous identification and serotyping of Actinobacillus pleuropneumoniae serotypes 2, 5, and 6.
Serotype-specific DNA regions involved in the biosynthesis of capsular polysaccharides (cps region) were used to develop a multiplex PCR test for the simultaneous species identification and serotyping of Actinobacillus pleuropneumoniae serotypes 2, 5, and 6. Primers specific for serotypes 2, 5, and 6 were combined with the already existing species-specific primers used in a PCR test based on th...
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Serotyping of Actinobacillus pleuropneumoniae is based on detection of the serotype-specific capsular antigen. However, not all isolates can be serotyped, and some may cross-react with multiple serotyping reagents. To improve sensitivity and specificity of serotyping and for early detection, a multiplex PCR assay was developed for detection of A. pleuropneumoniae and identification of serotype ...
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A total of 245 isolates of Actinobacillus pleuropneumoniae included in this study were isolated from lung tissues of pigs which died of porcine pleuropneumonia. The isolates were obtained from 52 swine herds in the Czech Republic over the period 2003–2004. All the serotypes were serotyped by the coagglutination test. All isolates with cross-reactivity between serotypes 9 and 11 or 1, 9, and 11 ...
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ورودعنوان ژورنال:
- The Journal of veterinary medical science
دوره 72 5 شماره
صفحات -
تاریخ انتشار 2010